Electron paramagnetic resonance spectroscopy is often used in combination with optical detection of magnetic resonance (ODMR) techniques in studies of biochemical problems involving paramagnetic species - free radicals, metal ions and triplet excited molecules. Triplet spin labels are often used in a form of nitroxide biradicals and also as natural constituents of biopolymers that contain chromophores which can be excited to the triplet state. An impressive amount of such experimental information has been accumulated on photophysical properties of porphyrin, photosynthetic pigments and bacteriochlorophyll systems.
Fluorescence detection of magnetic
resonance (FDMR) is an especially useful technique, since the
phosphorescence is often very weak or even undetectable for biopolymers.
In KTH we have developed unique techniques
to calculate all spin-Hamiltonian parameters
which determine the ODMR /FDMR spectra. We can also establish the nature
of the ODMR line
broadening for triplet state signals and find
direct connections between the optical and magnetic properties of the
emitting chromophores. Comparative
calculations are made of the radiative lifetimes of the triplet state
spin sublevels
with account of intramolecular and intermolecular spin-orbit coupling effects
and of zero-field and other parameters of the spin-Hamiltonian
which provide useful
information on biopolymer triplet states.
You are welcome to contact Boris Minaev for
more information.
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